Tuna Bone Collagen Peptides Modulate Insulin Sensitivity via PI3K/AKT/GLUT4 Pathway in HepG2 Cells

Abstract

Background: Insulin resistance is a key metabolic disorder associated with type 2 diabetes and cardiovascular diseases, presenting significant challenges in clinical management. This study explores the potential of tuna bone collagen peptides (TBCP) to enhance insulin sensitivity in HepG2 cells through modulation of the PI3K/AKT/GLUT4 signaling pathway. Methods: HepG2 cells were treated with varying concentrations of TBCP, and cell viability, glucose uptake, and the expression levels of key proteins and genes related to glucose metabolism were assessed. Results: TBCP significantly enhanced insulin sensitivity by improving cell viability, promoting glucose uptake, and modulating the expression of PI3K, AKT, and GLUT4. Notably, TBCP upregulated the PI3K/AKT/GLUT4 pathway, indicating its role in enhancing insulin signaling. Conclusion: The findings suggest that TBCP could serve as a novel therapeutic agent for managing insulin resistance. This study provides valuable insights into the potential of marine-derived collagen peptides for improving metabolic health and supports the development of functional foods or therapeutics targeting insulin resistance.