The Effect of Wumei Pill on Atrogin-1, MURF1 in Diabetic Myopathy

Abstract

Objective: Explore the effects of Wumei Pill on FBX32 (Atrogin-1), MURF1 related to muscle atrophy in diabeticopathy. Methods: (1) Male SD rats at 6-8 weeks of age were randomly divided into control group, model group, metformin group, andumei Pill group. Except for the control group, the rest of the rats were injected intraperitoneally with streptozotocin to establish a diabetic model. () The blood glucose of rats was monitored weekly, and the body weight was measured. (3) At the end of the 8-week experimental period, the rats were fast overnight and fasted for 12 hours before sacrifice, and the weight was weighed. The gastrocnemius muscle and soleus muscle tissues of the rats were taken, part the soleus muscle tissue was fixed with paraformaldehyde, and the rest of the tissues were frozen for storage. (4) The morphology of the soleus muscle of was observed by HE staining method, and the cross-sectional area of its fibers was measured; the expression levels of Atrogin-1 and MURF1 proteins rat skeletal muscle tissue were detected by real-time fluorescent quantitative PCR experiment and protein immunoblotting experiment. Results: (1) Wumei Pill can effectively control the trend of weight loss and the rise in blood sugar in diabetic rats. (2) The cross-section area of the soleus muscle fibers in the Wumei Pill group was significantly higher than that in the model group; and the expression levels of two proteins, Fbx32 MURF1, in their skeletal muscle tissue were significantly lower. Conclusion: Wumei Pill prevents muscle fiber structure damage, and reduces the expression of Fbx32 and MURF1 protein, thus playing its role in preventing musclerophy in diabetic myopathy.