Effect of Sucrose Concentration on In Vitro Shoot Regeneration in Stevia rebaudiana Bertoni

Authors

  • Alexandre Anacleto Plant Tissue Culture Laboratory, Dr. Babasaheb Ambedkar Marathwada University, Chh. Sambhajinagar (MS), India
  • Libanio Xavier Fernandes Plant Pathology & Fungal Biotechnology Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Chh.Sambhajinagar (MS), India

DOI:

https://doi.org/10.53469/jrse.2025.07(11).12

Keywords:

Stevia rebaudiana, sucrose concentration, micropropagation, plant tissue culture, in vitro regeneration

Abstract

Sucrose serves as the primary carbon source in plant tissue culture, influencing cellular growth and development. The present study investigates the effect of varying sucrose concentrations on shoot regeneration in Stevia rebaudiana using nodal explants. Experiments were conducted on Murashige and Skoog (MS) medium supplemented with different sucrose levels. The highest shoot proliferation (19.6±0.452) and maximum shoot length (11.66±0.172 cm) were observed at 40 g/L sucrose concentration. The study highlights the significance of optimizing sucrose levels for enhanced in vitro propagation of Stevia rebaudiana.

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Published

2025-11-28

How to Cite

Anacleto, A., & Fernandes, L. X. (2025). Effect of Sucrose Concentration on In Vitro Shoot Regeneration in Stevia rebaudiana Bertoni. Journal of Research in Science and Engineering, 7(11), 56–57. https://doi.org/10.53469/jrse.2025.07(11).12

Issue

Vol. 7 No. 11 (2025)

Section

Articles

License

Copyright (c) 2025 Alexandre Anacleto, Libanio Xavier Fernandes

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


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